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KMID : 0861019990140010103
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Korea Journal of Herbology
1999 Volume.14 No. 1 p.103 ~ p.109
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Neuroprotective Effect of Gastrodiae Rhizoma on Dopaminergic Neuronal Cell Death in Parkinson´s disease
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金護哲/HoCheolKim
李福男/金涌植/李尙仁/BokNamLee/YongSikKim/SangInLee
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Abstract
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Parkinson´s disease is characterized by chronic progress of mesencephalic dopaminergic neuronal death. The activity of mitochondrial complex I of the dopaminergic neuron is reduced in Parkinson´s disease, and the reduction of mitochondrial complex I activity seems to play an improtant role in the degenerative process of the dopaminergic neuron. Methyl- 4- phenylpyridium ion (MPP) is taken up selectively into the dopaminergic neuron and causes reduction of mitochondrial complex I activity and dopaminergic neuronal death. Gastrodiae Rhizoma, the rhizoma of Gastrodia elata B_L, has been used for the effect of calming the liver in Tradicional Korean Medicine. This drug has especially used for the sign of tremr in Traditional Korean Medicine, which is one of the main simptom in Parkinson´s disease. Gastrodiae Rhizoma has reportedly antioxidant, glutamate antagonistic and GABA agonistic effects. We hypothesized that Gastrodiae Rhizoma has the neuroprotective effect on the dopaminergic cell death induced by MPP^+ treatment as a model of Parkinsonism. In our experiments, MPP^+ caused cytotoxicity mainly in the dopaminergic neuron of S.D. rat fetal mesencephalon in a dose- dependent manner. More than 0.5% MPP^+ showed reduction of the number of dopaminergic neuron. We investigated the neuroprotective effect of Gastrodiae Rhizoma after treatment with 0.5% MPP^+ and checked the dopamine and DOPAC level, dopamine uptake and TH cell number. At the dose of G. elata 20㎎/㎖, there is no significant protective effects in the sample group induced by 0.5% MPP^+ treatment. We conclude that G. elata has no neuroprotective effect on the cytotoxicity induced by MPP^+ in dopaminergic cell culture.
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KEYWORD
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